Glycogen metabolism in Novikoff ascites-hepatoma cells
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منابع مشابه
Glycogen metabolism in Novikoff ascites-hepatoma cells.
A study of the enzymes of the glycogen pathway in Novikoff ascites hepatoma shows that glycogen synthetase has the lowest activity and that the tumour contains no high-K(m) soluble glucokinase. However, incubation of tumour cells with metabolizable sugars in vitro, or intraperitoneal administration of glucose into the tumour-bearing rat, results in glycogen accumulation by the tumour cells. Gly...
متن کاملPasteur Effect in Novikoff Ascites-hepatoma Cells.
Inhibition of fermentation or of glucose uptake in the presence ofoxygen has been observed in many forms of life and is generally referred to as the 'Pasteur effect'. In most tissues, oxidative inhibition of fermentation (ethanol or lactate production) is reflected by a parallel decrease in glucose consumption. Since certain tissues utilize lactate significantly, estimation of the Pasteur effec...
متن کاملAssociation of exogenous RNA with Novikoff hepatoma ascites cells.
RNA taken up into the cells. The 4 types of RNA used included whole cellular 4 5, 18 5, and 28 S RNA from NHAC and the very GC-rich B3 fragment enzymatically cleaved from 28 S RNA of NHAC (1 7, 37, 37). The B3 fragment, composed of approximately 550 nucleotides, was chosen because it is highly hydrogen bonded and is less susceptible to degradation by RNase. The present results show that the cel...
متن کاملPurification of thioredoxin from rat Novikoff ascites hepatoma.
Partial purification of a thioredoxin system from Novikoff ascites hepatoma cells has been previously reported (MOORE, E. C. (1967) Biochem. Biophys. Res. Commun. 29, 264-268). Thioredoxin from the same mammalian source has now been purified to electrophoretic homogeneity by ammonium sulfate fractionation, heat treatment, DEAE-cellulose chromatography, and Sephadex chromatography. 1-Dimethylami...
متن کاملDifferences in nucleolar antigens of rat liver and Novikoff hepatoma ascites cells.
Antisera to nucleoli of Novikoff hepatoma ascites and normal rat liver cells were produced in rabbits by injection of whole, isolated nucleoli. These antisera have been used to compare the nucleolar antigens that were partially fractionated by differential solubilization from nucleoli. Fourteen antigens were detected by these antisera; ten of these antigens were detected by both antisera. Oucht...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1967
ISSN: 0006-2936
DOI: 10.1042/bj1020468